Surfacine prevents bio-film formation.

The ability of Surfacine treated material to resist biofilm formation was evaluated by subjecting Surfacine treated and untreated polyurethane tubes to conditions encouraging biofilm growth, followed by exposure to organism mixture Pseudomonas diminuta (ATCC#19146), Pseudomonas aeruginose (ATCC#9027), Klebsiella pneumoniae (ATCC#13883), Bacillus cereus (ATCC#11778), Escherichia coli (ATCC#8739) and Enterococcus faecalis (ATCC#19433).

Within 24 hours, microorganisms in untreated tubes had grown from an initial concentration of 104 cfu/mL to an average of 3x105 cfu/mL. Surfacine treated tubes had no viable micro-organisms.

The tubes were then washed and refilled with water. Eight days later, the untreated tubes still yielded 105 cfu/mL (resulting from the biofilm established during the first day of incubation) while the Surfacine treated tubes yielded no microorganisms.